The group recorded the instrumental track - which is 14 minutes and 57 seconds long - back in 1966 and whilst a shorter version was released on their debut album 'The Piper at the Gates of Dawn', this will be the first time the band have released this version.
To celebrate Record Store Day 2017 on Saturday, April 15, the band will release the one-sided 12" 180-gram black vinyl, which will play at 33 1/3 RPM. The vinyl will also come with a fold-out poster and an A6 postcard of a picture of the band taken whilst they were working on their debut single 'Arnold Layne'.
'Interstellar Overdrive' was written and recorded by Syd Barrett, Roger Waters, Richard Wright and Nick Mason at the Thomson studio in Hemel Hempstead, Hertfordshire on 31 November 1966.
Fans will also have a chance to purchase the vinyl from the Pink Floyd: Their Mortal Remains Exhibition at London's Victoria & Albert Museum from May 13 for a limited time.
Meanwhile, Pink Floyd legends Roger Waters and Nick Mason previously revealed they are both up for headlining Glastonbury - but not together.
Speaking during a Q&A session, Roger said: "I did Glastonbury once, I think. It was really cold. But there were a lot of people and it seemed very jolly and I liked it. Yeah, I would do it again."
Speaking about his ambition to top the bill on the Pyramid Stage, Nick added: "It would be nice to add it to the list of things. I've never played Glastonbury. It would be fun to do it. I don't think it would be very likely."