The Chinese firm had a reprieve which allowed some exceptions to a trade ban after Donald Trump's administration labelled the company a security threat.
Under the terms of the temporary general license, software providers in the United States could continue to send updates and patches to older devices.
As reported by the Washington Post, the Commerce Department confirmed the license had now expired.
It added that the agreement had given "an opportunity for users of Huawei devices and telecommunications providers to continue to temporarily operate such devices and existing networks while hastening the transition to alternative suppliers".
A spokesman for Huawei added that the firm is "monitoring the situation and assessing the potential impact".
Until now, the license - which expired on August 13 - had previously allowed Google to provide Android updates for older devices which were sold before May 16, 2019, but this is no longer possible.
With the reprieve now over, it means Google isn't authorised to give updates to Huawei, or for the latter to push updates to apps such as Gmail and Google Maps.
However, the full impact of the change isn't clear just yet, as devices won't immediately stop working as a result.