The England manager has named a 35-man squad for the upcoming games against Uruguay on March 27 and Japan on March 31, and he's picked a larger group of players to "open up the picture" and increase competition for places.
He said: "We decided to divide it into basically two camps almost, so we bring players in that we haven't seen, who haven't played so much to open up the picture, and the competition for plane tickets to the US.
"Then from Friday and Saturday, a group of players will come into camp - ten or eleven players who get a rest before and we will then go with a new group and mix of players into the match against Japan."
As well as Manchester United duo Mainoo and Maguire, Newcastle United's Lewis Hall has earned a recall.
His Magpies teammates Aaron Ramsdale, Anthony Gordon and Tino Livramento are also involved this time after missing out on Tuchel's last England squad.
Others included this month who were omitted last time are Jason Steele (Brighton and Hove Albion), Fikayo Tomori (AC Milan), James Garner (Everton), Dominic Calvert-Lewin (Leeds United), Noni Madueke (Arsenal), Cole Palmer (Chelsea) and Dominic Solanke (Tottenham Hotspur).
Real Madrid defender Alexander-Arnold has been snubbed despite being a regular starter for his team.
From Tuchel's November squad, Chelsea duo Trevoh Chalobah and Reece James have been dropped, along with AFC Bournemouth midfielder Alex Scott.
England have picked five goalkeepers in the squad, including Brighton's uncapped shot-stopper Jason Steele, who is intended to be included as a training goalkeeper for the 2026 World Cup.
Thomas Tuchel England squad in full for March 2026 fixtures:
Goalkeepers:
Dean Henderson (Crystal Palace), Jordan Pickford (Everton), James Trafford (Manchester City), Aaron Ramsdale (Newcastle United), Jason Steele (Brighton and Hove Albion)
Defenders:
Dan Burn (Newcastle United), Marc Guéhi (Manchester City), Lewis Hall (Newcastle United), Ezri Konsa (Aston Villa), Tino Livramento (Newcastle United), Harry Maguire (Manchester United), Nico O'Reilly (Manchester City), Jarell Quansah (Bayer Leverkusen), Djed Spence (Tottenham Hotspur), John Stones (Manchester City), Fikayo Tomori (AC Milan)
Midfielders:
Elliot Anderson (Nottingham Forest), Jude Bellingham (Real Madrid), James Garner (Everton), Jordan Henderson (Brentford), Kobbie Mainoo (Manchester United), Declan Rice (Arsenal), Morgan Rogers (Aston Villa), Adam Wharton (Crystal Palace)
Forwards:
Jarrod Bowen (West Ham United), Dominic Calvert-Lewin (Leeds United), Eberechi Eze (Arsenal), Phil Foden (Manchester City), Anthony Gordon (Newcastle United), Harry Kane (Bayern Munich), Noni Madueke (Arsenal), Cole Palmer (Chelsea), Marcus Rashford (Barcelona, loan from Manchester United), Bukayo Saka (Arsenal), Dominic Solanke (Tottenham Hotspur)
