The German city triumphed in a survey carried out by Hostelworld, which asked residents of 41 cities across 27 countries how they would rate the nightlife in their home cities.
Each of the 4,100 surveyed were asked to rate their city based on the quality of the nightlife, such as a variety of things to do and its general enjoyment, as well how welcoming the residents are and how safe people feel and how easy the transport is there.
Copenhagen, Denmark; Berlin, Germany and Dublin, Ireland all finished in the top four spots whilst New York, United States managed an impressive tenth place.
However, the British capital of London only managed to rank in 26th place owing to its unaffordability.
Marek Mossakowski, Global Head of Brand at Hostelworld, said: "We scored a 'night out' in global cities based on five key factors. We looked at the overall quality of things to do, the openness of the locals, how safe people feel, getting around the city, and affordability.
"Hamburg scored highly across the board, but particularly well when looking at how easy it is to travel around the city and how open the locals are, which includes how easy it is to meet new people and how welcoming the residents are to tourists ...
"The results of this study have been fascinating, especially the performance of smaller cities - most notably with Hamburg taking top spot over Berlin. The results of this study have been fascinating, especially the performance of smaller cities - most notably with Hamburg taking top spot over Berlin."