The Real Madrid player appeared to gesticulate with his arms that he did not want to be substituted, when he was taken off for Aston Villa star Morgan Rogers with just six minutes to go, and the England manager has insisted players need to show "respect" towards their teammates.
He told BBC Radio 5 Live: "I don't want to make more out of it, but I stick to my words - behavior is key, and respect towards the team-mates who come in.
"Decisions are made and you have to accept it as a player.
"Morgan Rogers was for sure not happy when he couldn't start today because he deserves to play for us and he wants to play all the time. We gave him a bit of a rest because he came with a lot of minutes for his club and played against Serbia."
Bellingham was starting his first England game since June when he lined up in Tirana, and Tuchel admitted he knows the star "was not happy".
He said: "I have to review it.
"I saw he was not happy, I don't want to make it bigger at the moment than it is.
"My words stand, we are about standards, level and commitment to each other and respect to each other. We will not change or decision just because someone waves their arms."
In August, Tuchel apologized for previously describing Bellingham's on-field behavior as "repulsive".
The ex-Chelsea manager said: "I used this word unintentionally, just to make it very clear.
"There was no message. There was no hidden agenda.
"I fully understood and understand that it's my responsibility that I created these headlines. I am sorry for the upset and I am sorry for the headlines I created.
"I am experienced enough and should've known better, I should've done better. I thought I had a little more credit with you guys (media) that I do all this in my second language.
"I did it the morning after a loss, with not a lot of sleep, in a live interview, and I used the wrong word. Again, it's my responsibility."