The world-famous firm is opening the store in New York and has announced that it will sell a range of different products, including toys, that have a rating of four or more.
It's been confirmed, too, that consumers will pay less provided they are already subscribed to Amazon's Prime service.
The stores are an important part of Amazon's long-term business ambitions.
Earlier this month, meanwhile, the company announced that it had expanded its grocery delivery service.
The online marketplace already offered a service in 28 US cities in which Prime customers can place a grocery order with their local Whole Foods store, and have Amazon deliver them their food.
Now, Amazon - which acquired Whole Foods Market for $13.7 billion in 2017 - expanded the service to a further 10 cities, including Charlotte in North Carolina, Las Vegas in Nevada, Memphis and Nashville in Tennessee, New Orleans in Louisiana, Oklahoma City in Oklahoma, Phoenix and Tucson in Arizona, Raleigh in North Carolina, and Seattle in Washington.