The world number 29 - who was once as high as third in the world rankings - brought the former Wimbledon champion on board in May in a bid to arrest his alarming slide in form over the past couple of years but has now confirmed that the pair have parted ways.
Tsitsipas wrote on social media: "Working with Goran Ivanisevic was a brief but intense experience and a truly valuable chapter in my journey.
"I’m thankful for the time, effort, and energy he dedicated to me and my team.
"As we are now following our separate ways, I have only respect for Goran - not just for what he’s achieved in tennis, but also for who he is as a person.
"I wish him nothing but the very best moving forward."
Ivanisevic issued a damning verdict on the Greek's fitness after he retired from his first-round match at Wimbledon and later admitted that the former French Open finalist had been "affected" by his strong words.
He told Croatian media: "Well, it affected him a little because every tennis player, every athlete, when their ego gets a little inflated, doesn't like criticism.
"Anyway, I told him: tennis is an individual sport. To play on the court, you have to be physically and mentally prepared. He wasn't mentally or physically prepared at Wimbledon. Back problems, a million problems. So it's a long road, there's no magic wand, no magic tricks, you have to work."
Tsitsipas's form has declined in recent years due to a series of injury problems and he has only managed one victory at a Grand Slam so far this year.